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HEK 293 cells were treated with Ser/Thr phosphatase inhibitor,calyclinA (100 nM) for 30 min, or Tyr phosphatase inhibitor, pervanadate (1 mM) for 30 mim, and then lyzed in SDS-PAGE loading buffer.
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| Reference | None |
|---|---|
| Composition of gel | Zn2+ -Phos-tag: 25µM Polyacrylamide: 8.5%(w/v) |
| Detection | WB: anti-CDK2 |